8 endotoxin

Bacterial Endotoxins and their Impact on Respiratory System among Greek Cotton Industry Workers

1Department of Respiratory Medicine, University of Thessaly Medical School, Biopolis, Larissa 41110, Greece

2Department of Hygiene and Epidemiology, Faculty of Medicine, University of Thessaly, Larissa 41222, Greece

Correspondence to
Ioannis D Anyfantis, Department of Respiratory Medicine, University of Thessaly Medical School, Biopolis, Larissa 41110, Greece

E-mail: yiannis4@gmail.com

Received: Jan 19, 2017

Accepted: Jan 29, 2017

Exposure to cotton dust is associated with several occupational lung diseases. The dust is often contaminated with Gram-negative bacteria containing endotoxins.1-4 This article is a secondary analysis from a cross-sectional study on respiratory symptoms and lung function of cotton industry workers in Greece. Details on the assessment of respiratory symptoms and lung function indices have been reported elsewhere.5

To measure airborne endotoxins, 37-mm endotoxin-free fiberglass filters were used. For each measurement, the whole air sampling head was decontaminated using depyrogenation procedures. A dust sampling head attached to a Gillian high-flow pump (Model HFS513A, Sensidyne, USA) was used; the airflow was set to 2.0 L/min. Each single measurement lasted four hours. Non-pyrogenic tools were used to place each sample at a sealed container and prepare them for transport. The filters were then sent to University of Thessaly, at the Regional Laboratory of Public Health for endotoxin analysis.

Endotoxin levels were measured using Limulus Amebocyte Lysate (LAL) endpoint method (Regional Public Health Laboratory, University of Thessaly, Katsigras Building, 41222, Larissa, Greece). The LAL test performed was qualitative, indicating endotoxin level above specific thresholds or not. Air particulates collected on filters were extracted by shaking filters in pyrogen-free tubes with 5-mL endotoxin-free water. By consequent dilutions, four different endotoxin concentrations were made. The endotoxin level was expressed as endotoxin units per cubic meter of air volume (EU/m3) and categorized into “<100,” “100–1000,” “1000–2000,” and “>2000” EU/m3.

For the time being, there is only one recommendation on endotoxin exposure limit (an average of 90 EU/m3 over an 8-hour working day) proposed by the Health Council of the Netherlands.6 High exposure levels were recorded in our study, near the ginning machines (>2000 EU/m3) and the blending machines—before the spinning process (>2000 EU/m3).

Statistical analysis revealed a significant (p=0.02) linear correlation between endotoxin levels and FEV1/FVC among studied workers. However, in order to estimate the level of cumulative exposure to endotoxins, a new variable “endotoxin exposure” (EXP) was defined as multiplication of “working years at a certain position in cotton industry” by “respective endotoxin exposure level measured at that working position.” EXP can vary from ‘0’ to ‘3.’

Multiple linear regression analysis showed that FEV1/FVC% can be predicted according to the following equation:

299462.png

It was found that exposure to high levels of airborne endotoxin (>2000 EU/m3) was significantly (p<0.001) associated with reduced spirometry scores. Endotoxin exposure was also found to be highly correlated with presence of dyspnea (p<0.001), chronic cough (p=0.016), and spitting (p<0.001).

Measuring endotoxin levels may be a use­ful tool for the prevention of obstructive respiratory disorders among cotton industry workers. However, there is no consensus on the threshold concentration of endotoxin at workplace.

Conflict of Interest: None declared.

References

  1. Oldenburg M, Latza U, Baur X. Exposure-response relationship between endotoxin exposure and lung function impairment in cotton textile workers. Int Arch Occup Environ Health 2007;80:388-95.
  2. Mayan O, Torres Da Costa J, Neves P, et al. Respiratory effects among cotton workers in relation to dust and endotoxin exposure. Ann Occup Hyg 2002;46:277-80.
  3. Williams LK, Ownby DR, Maliarik MJ, Johnson CC. The role of endotoxin and its receptors in allergic disease. Ann Allergy Asthma Immunol 2005;94:323-32.
  4. Paudyal P, Semple S, Niven R, et al. Exposure to Dust and Endotoxin in Textile Processing Workers. Ann Occup Hyg 2011;55:403-09.
  5. Anyfantis ID, Rachiotis G, Hadjichristodoulou C, Gourgoulianis KI. Respiratory Symptoms and Lung Function among Greek Cotton Industry Workers: A Cross-Sectional Study. Int J Occup Environ Med 2017;8:32-38.
  6. Dutch Expert Committee on Occupational Standards. Endotoxins: health-based recommended occupational limit. The Hague: The Health Council of the Netherlands; 2010. Report No: Publication No. 2010/04OSH.

Cite this article as: Anyfantis ID, Rachiotis G, Hadjichristodoulou C, Gourgoulianis KI. Bacterial endotoxins and their impact on respiratory system among Greek cotton industry workers. Int J Occup Environ Med 2017;8:-126.




 pISSN: 2008-6520
 eISSN: 2008-6814

Creative Commons LicenseThis work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License